Directed Evolution of Substrate-Optimized GroEL/S Chaperonins
نویسندگان
چکیده
منابع مشابه
Directed Evolution of Substrate-Optimized GroEL/S Chaperonins
GroEL/S chaperonin ring complexes fold many unrelated proteins. To understand the basis and extent of the chaperonin substrate spectrum, we used rounds of selection and DNA shuffling to obtain GroEL/S variants that dramatically enhanced folding of a single substrate-green fluorescent protein (GFP). Changes in the substrate-optimized chaperonins increase the polarity of the folding cavity and al...
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ژورنال
عنوان ژورنال: Cell
سال: 2002
ISSN: 0092-8674
DOI: 10.1016/s0092-8674(02)01198-4